Identification

Title

Microbial activity and carbon fluxes in rainforest soil – Tsunami Hill, Calvert Island – June 2015 - April 2016

Alternative title(s)

Seasonal and ecohydrological regulation of active microbial populations involved in DOC, CO2 and CH4 fluxes in temperate rainforest soil

Abstract

This dataset is designed to elucidate the microbial drivers of carbon fluxes from deep ombrotrophic peat bog and bog forest ecosystem comparison plots (ECP) on Calvert Island as part of the Kwakshua Watersheds Program (KWP). This dataset was collected between June 2015 and April 2016 at the Tsunami Hill ECP sites. It combines: -Soil chemistry (total carbon, total nitrogen, NH4-N, NO3N, total sulphur, available PO4-P, pH, cations) -Micrometeorology (precipitation, air temperature, soil temperature, soil redox potential, soil gravimetric water content, water table depth) -Soil water chemistry (dissolved organic carbon (DOC), specific absorbance of DOC at 254nm (SUVA254), cations), -Soil gas flux measurements (CO2, CH4, N2O), -Fungal ITS, Eukaryotic ribosomal small-subunit (SSU) amplicon sequences from DNA and RNA, prokaryotic SSU amplicon sequences from DNA and RNA -Metatranscriptomes Detailed information regarding field names and descriptions are available with the dataset through the link below. Basic methods are outlined in the supplemental information section of this record and detailed methods are available in the associated publication (Levy-Booth et al. 2018) Cite as: Levy-Booth, D.J, I.J.W. Giesbrecht, C.T.E. Kellogg, T.J. Heger, D.V. D’Amore, P.J. Keeling, S.J. Hallam, W.W. Mohn (2018). Seasonal and ecohydrological regulation of active microbial populations involved in DOC, CO2 and CH4 fluxes in temperate rainforest soil. Version 1.0. Hakai Institute. Dataset. doi: 10.21966/1.715630. [access date]. DOI: 10.21966/1.715630

Resource type

dataset

Resource locator

https://drive.google.com/file/d/1soGAiNLC1kqpYAPOi2ksX7_c3GcbJoEA/view?ts=5a9d9a60

protocol: WWW:LINK-1.0-http--link

name: Levy-Booth et al. 2018. Soil chemistry data

description: Data used in the analysis presented in Levy-Booth et al. 2018

function: download

Unique resource identifier

code

codeSpace

Dataset language

eng

Spatial reference system

code identifying the spatial reference system

Additional information source

Basic data collection methods: Soil chemistry: Sampled using peat-corer. Analyzed at UBC and by BC Analytical Laboratory Micrometeorology: Continuous data collection using remote sensors logging directly to database Soil water: Sampled using piezometer and lysimeter. Analyzed by BC Analytical Laboratory Gas flux rates: Headspace gas samples collected from in situ mixed chambers and measured using trace gas chromatography at UBC SSU amplicon sequences: DNA and RNA extracted from soil samples, amplified for ITS, 18S rRNA and 16S rRNA V4 regions, sequenced and deposited in European Nucleotide Archives (ENA links provided). For detailed methods see associated publication: Levy-Booth et al., 2018

Classification of spatial data and services

Topic category

environment

biota

Keywords

Keyword set

keyword value

Downloadable Data

originating controlled vocabulary

Keyword set

keyword value

Soil

Terrestrial

Carbon

Microbial

Ecology

Peat

Bog

Forest

Sequencing

Nitrogen

Phosphorus

Sulphur

pH

Water

DOC

Cations

Temperature

Precipitation

Redox

Fungi

ITS

Eukaryotes

18s

Bacteria

Archaea

16s

SSU

Amplicon

iTag

Metatranscriptone

DNA

RNA

Gas

Flux

CO2

CH4

N2O

Geographic location

West bounding longitude

-128.13650264

East bounding longitude

-128.12495841

North bounding latitude

51.65523482

South bounding latitude

51.64966999

Temporal reference

Temporal extent

Begin position

2015-06-18

End position

2016-04-16

Dataset reference date

date type

creation

effective date

2017-01-22

date type

publication

effective date

2017-11-19

Frequency of update

Quality and validity

Lineage

Conformity

Data format

name of format

xlsx

version of format

1.0

Constraints related to access and use

Constraint set

Use constraints

Appropriate credit must be given to Hakai Institute and the authors of the dataset.

Responsible organisations

Responsible party

organisation name

University of British Columbia

full postal address

4520 - 2350 Health services Mall

Vancouver

V6t 1Z3

Canada

email address

dlevyboo@mail.ubc.ca

responsible party role

author

Metadata on metadata

Metadata point of contact

organisation name

Hakai Institute

full postal address

1002 Wharf Street - Suite 100

Victoria

V8W 1T4

telephone number

+1 (250) 590 3306

email address

data@hakai.org

responsible party role

publisher

Metadata date

2018-10-17T18:34:53

Metadata language

eng