Hakai Bulk and Size-Fractionated Chlorophyll and Phaeopigment Concentrations Collected by Niskin Bottle, Research

This dataset is comprised of Hakai Institute chlorophyll and phaeopigment concentrations collected from 2017 to present at stations within the Calvert Island, Johnstone Strait, and the Quadra Island study regions. Samples for these data were collected in the field using Niskin bottles. Sample water (250 ml) was filtered through: 1) a single glass fiber filter (GF/F, nominal pore size 0.7 um) to comprise a bulk measure of phytoplankton chlorophyll and phaeopigment concentrations and 2) a stack of filters (GF/F, 3 um, and 20 um polycarbonate filters) to estimate chlorophyll and phaeopigment concentrations from pico, nano, and micro-phytoplankton, respectively. Filters were extracted for 24 hours in 10 ml of 90% acetone and analyzed on Turner Designs Trilogy laboratory fluorometers using the acidification module and following the method of Holm Hansen et al. (1965).

Data have been quality controlled by manual inspection which included a comparison of bulk concentrations against the sum of the size-fractionated filter concentrations. Where available, further comparisons were done with other measures of chlorophyll concentrations including in situ CTD chlorophyll fluorescence and high-performance liquid chromatography (HPLC) data.

Data were collected by the Hakai Institute Oceanography and Nearshore programs.

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Licence: CC-BY-4.0

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Metadata Reference Date(s) October 23, 2025 (Creation)
October 23, 2025 (Revision)
Frequency of Update As Needed

Responsible Party 1
Affiliation
Hakai Institute ROR logo
Role
Distributor

Field Value
Ocean Variables Other
Scope Dataset
Status On Going
Topic Category oceans
Maintenance Note Generated from https://hakaiinstitute.github.io/hakai-metadata-entry-form#/en/hakai/7U7b8oPpeTN6gjvXlUCTGJr5pga2/-MadZvZJ4ZeikpCgRcV8
Spatial Extent { "coordinates": [ [ [ -128.5, 52.27 ], [ -127.4, 52.21 ], [ -127.2, 51.66 ], [ -125.6, 51.13 ], [ -124.8, 50.96 ], [ -124.1, 50.43 ], [ -124.7, 49.98 ], [ -124.9, 49.8 ], [ -126.7, 50.45 ], [ -128.1, 51.37 ], [ -128.4, 51.69 ], [ -128.5, 52.27 ] ] ], "type": "Polygon" }
North Bounding Latitude 49.8
South Bounding Latitude 52.27
East Bounding Longitude -124.1
West Bounding Longitude -128.5
Default Locale English
Projects
  1. Oceanography
Included in Data Catalogue
Included in Data Catalogue 1
Name
Hakai Data Catalogue
Description
Science on the Coastal Margin
URL
https://catalogue.hakai.org