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   set  data   graph     High performance liquid chromatography phytoplankton pigment timeseries for the northern
Salish Sea and central coast, British Columbia
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String TimeSeriesProfile
attribute NC_GLOBAL cdm_profile_variables String project,survey,source,time
attribute NC_GLOBAL cdm_timeseries_variables String work_area,site_id
attribute NC_GLOBAL comment String ##Limitations:
Care should be taken when using HPLC phytoplankton pigment concentrations for community based analysis. Species within different phytoplankton groupings can show comparable pigment profiles or variable presence/absence of marker pigments. Pigment concentrations can also vary as a result of environmental conditions (light, nutrients, etc.). In addition, small cryptic species with similar pigment profiles as broader groupings are often not separated and are included in the biomass of the most similar grouping. Furthermore, endosymbiosis can result in mixotrophic grazers displaying pigment profiles of their endosymbiont (i.e. cryptophyte pigments within the ciliate Mesodinium rubrum). These issues can result in misclassifications or error when using chemotaxonomic and other pigment based methods. Strong knowledge of expected phytoplankton species is required prior to analysis and outputs are meant to represent broad trends in phytoplankton species composition.
attribute NC_GLOBAL contributor_name String Del Bel Belluz, Justin;Jordison, Drew;Evans, Wiley;Hakai Institute;Tula Foundation
attribute NC_GLOBAL contributor_role String custodian,author,editor,pointOfContact,principalInvestigator,originator;custodian,pointOfContact,contributor;custodian,principalInvestigator;publisher,owner,distributor;funder
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_city String Campbell River
attribute NC_GLOBAL creator_country String Canada
attribute NC_GLOBAL creator_institution String Hakai Institute
attribute NC_GLOBAL creator_name String Hakai Institute
attribute NC_GLOBAL creator_ror String https://ror.org/02pry0c91 (external link)
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.hakai.org/ (external link)
attribute NC_GLOBAL date_modified String 2024-07-22T20:18:57.436Z
attribute NC_GLOBAL doi String https://doi.org/10.21966/bw2d-tg65 (external link)
attribute NC_GLOBAL featureType String TimeSeriesProfile
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL history String Metadata record history:
- additionalDocumentation:
- authority: URL
code: https://ntrs.nasa.gov/citations/20110008482
title:
en: The Fourth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-4)
fr: "La quatri\xE8me exp\xE9rience Round Robin d'analyse HPLC de SeaWiFS (SeaHarre-4)"
translations:
fr:
message: "text translated using the Amazon translate service / texte traduit\
\ \xE0 l'aide du service de traduction Amazon"
verified: false
scope: Dataset
statement:
en: Explanation of HPLC USC analysis method and comparison with other methods
fr: "Explication de la m\xE9thode d'analyse HPLC USC et comparaison avec d'autres\
\ m\xE9thodes"
translations:
fr:
message: "text translated using the Amazon translate service / texte traduit\
\ \xE0 l'aide du service de traduction Amazon"
verified: false
attribute NC_GLOBAL id String 7fd3ec6c-083a-4942-84b4-215e69492072
attribute NC_GLOBAL infoUrl String https://catalogue.hakai.org/dataset/ca-cioos_7fd3ec6c-083a-4942-84b4-215e69492072
attribute NC_GLOBAL institution String Hakai Institute
attribute NC_GLOBAL keywords String abundance and biomass, British Columbia, Canada, chlorophyll a, CIOOS:phytoplanktonBiomassAndDiversity, climate change, coastal zone, hplc, phytoplankton
attribute NC_GLOBAL keywords_vocabulary String CIOOS: CIOOS Essential Ocean Variables Vocabulary
attribute NC_GLOBAL license String https://creativecommons.org/licenses/by/4.0 (external link)
attribute NC_GLOBAL metadata_form String https://hakaiinstitute.github.io/hakai-metadata-entry-form#/en/hakai/7U7b8oPpeTN6gjvXlUCTGJr5pga2/-O25qzW3OpioeACGpRHl (external link)
attribute NC_GLOBAL metadata_link String https://catalogue.hakai.org/dataset/ca-cioos_7fd3ec6c-083a-4942-84b4-215e69492072
attribute NC_GLOBAL naming_authority String ca.cioos
attribute NC_GLOBAL product_version String 1.0
attribute NC_GLOBAL progress String onGoing
attribute NC_GLOBAL project String Oceanography
attribute NC_GLOBAL publisher_city String Campbell River
attribute NC_GLOBAL publisher_country String Canada
attribute NC_GLOBAL publisher_institution String Hakai Institute
attribute NC_GLOBAL publisher_name String Hakai Institute
attribute NC_GLOBAL publisher_ror String https://ror.org/02pry0c91 (external link)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL publisher_url String https://www.hakai.org/ (external link)
attribute NC_GLOBAL sourceUrl String (source database)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v70
attribute NC_GLOBAL subsetVariables String project,work_area,survey,site_id,source
attribute NC_GLOBAL summary String High performance liquid chromatography (HPLC) is a method that quantifies concentrations of phytoplankton pigments from bulk water samples. Phytoplankton species groupings (i.e. diatoms, dinoflagellates, etc.) typically contain different pigments, and concentrations of pigments, used for photosynthesis. These differences make it possible to use statistical methods (e.g. chemotaxonomic analysis, CHEMTAX) to estimate phytoplankton group-level biomass contributions within a bulk water sample. These methods provide unique and valuable insight into phytoplankton community dynamics as they quantify the full phytoplankton size-spectrum, are relatively fast and cost-effective and, are directly relatable to remote sensing (i.e. pigments drive differences in light measured by satellites). Furthermore, HPLC is considered the gold-standard for quantifying phytoplankton total chlorophyll a concentrations (TChla, proxy for bulk phytoplankton biomass) required for satellite remote sensing validation.

The Hakai institute has been collecting HPLC samples in the Northern Salish Sea (NSS) at the QU39 long-term ecological research station (LTER) and at various stations on the central coast of British Columbia since 2015. These data are used to monitor phytoplankton group level dynamics, build knowledge of different environmental conditions driving their variability (Del Bel Belluz et al., 2021), investigate linkages to the food web and carbonate system, evaluate long-term change and, to build regional remote sensing models (Vishnu et al., 2022). Data from station QU39 within the NSS are collected weekly and from 2015 -2019 at 5m depth and afterwards at 0, 5 , 10 and 20m depth. On the central coast of British Columbia, data are collected monthly at 5m depth.

Samples are analyzed at the University of South Carolina Baruch Institute for Marine and Coastal Sciences using the USC method. This method was evaluated and is detailed in the NASA Fourth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-4, Hooker et al., 2010).

Phytoplankton form the base of marine food webs and play key roles in biogeochemical cycling and carbon sequestration. The high turnover rates of phytoplankton species make them ideal sentinels of environmental change as they quickly respond to perturbations; however, a paucity of data, notably in terms of community composition, exists across coastal systems hindering the derivation of baseline conditions to assess change. This knowledge gap is especially pertinent when considering that coastal regions are experiencing rapid climate-driven change including increased temperature and acidification, reduced oxygen and altered freshwater dynamics. The synergy of these influences has the potential to alter phytoplankton community and size structure having large downstream implications on ecosystem resiliency, food production and climate regulation.
attribute NC_GLOBAL title String High performance liquid chromatography phytoplankton pigment timeseries for the northern Salish Sea and central coast, British Columbia
variable project   String  
attribute project long_name String Project
variable work_area   String  
attribute work_area long_name String Work Area
variable survey   String  
attribute survey long_name String Survey
variable site_id   String  
attribute site_id cf_role String timeseries_id
attribute site_id long_name String Site Id
variable latitude   float  
attribute latitude _CoordinateAxisType String Lat
attribute latitude axis String Y
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   float  
attribute longitude _CoordinateAxisType String Lon
attribute longitude axis String X
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable source   String  
attribute source long_name String Source
variable project_specific_id   String  
attribute project_specific_id long_name String Project Specific Id
variable hakai_id   String  
attribute hakai_id long_name String Hakai Id
variable line_out_depth   double  
attribute line_out_depth long_name String Line Out Depth
attribute line_out_depth units String m
variable pressure_transducer_depth   double  
attribute pressure_transducer_depth long_name String Pressure Transducer Depth
attribute pressure_transducer_depth units String m
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth axis String Z
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth positive String down
attribute depth source String pressure_transducer_depth if available otherse line_out_depth
attribute depth standard_name String depth
attribute depth units String m
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time axis String T
attribute time cf_role String profile_id
attribute time ioos_category String Time
attribute time long_name String Collected
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time units String seconds since 1970-01-01T00:00:00Z
variable preserved   double  
attribute preserved ioos_category String Time
attribute preserved long_name String Preserved
attribute preserved standard_name String time
attribute preserved time_origin String 01-JAN-1970 00:00:00
attribute preserved units String seconds since 1970-01-01T00:00:00Z
variable analyzed   double  
attribute analyzed ioos_category String Time
attribute analyzed long_name String Analyzed
attribute analyzed standard_name String time
attribute analyzed time_origin String 01-JAN-1970 00:00:00
attribute analyzed units String seconds since 1970-01-01T00:00:00Z
variable acidified   byte  
attribute acidified long_name String Acidified
variable volume   double  
attribute volume long_name String Volume
attribute volume units String ml
variable lab_technician   String  
attribute lab_technician long_name String Lab Technician
variable filter_portion   double  
attribute filter_portion long_name String Filter Portion
variable pre_weight_mg   double  
attribute pre_weight_mg long_name String Pre Weight
attribute pre_weight_mg units String mg
variable post_weight_mg   double  
attribute post_weight_mg long_name String Post Weight
attribute post_weight_mg units String mg
variable filter_size_mm   double  
attribute filter_size_mm long_name String Filter Size
attribute filter_size_mm units String mm
variable filter_type   String  
attribute filter_type long_name String Filter Type
variable extraction_duration   String  
attribute extraction_duration long_name String Extraction Duration
attribute extraction_duration units String minutes
variable extraction_method   String  
attribute extraction_method long_name String Extraction Method
variable initial_extraction_volume   double  
attribute initial_extraction_volume long_name String Initial Extraction Volume
attribute initial_extraction_volume units String ml
variable volume_filtered_ml   double  
attribute volume_filtered_ml long_name String Volume Filtered Ml
attribute volume_filtered_ml units String ml
variable final_extraction_volume   double  
attribute final_extraction_volume long_name String Final Extraction Volume
attribute final_extraction_volume units String ml
variable tac_peak   double  
attribute tac_peak long_name String Tac Peak
variable a_19_but   double  
attribute a_19_but long_name String 19 But
attribute a_19_but standard_name String mass_concentration_of_19_butanoyloxyfucoxanthin_in_sea_water
attribute a_19_but units String kg/m^3
variable a_19_hex   double  
attribute a_19_hex long_name String 19 Hex
attribute a_19_hex standard_name String mass_concentration_of_19_hexanoyloxyfucoxanthin_in_sea_water
attribute a_19_hex units String kg/m^3
variable alpha_carotene   double  
attribute alpha_carotene long_name String Alpha Carotene
attribute alpha_carotene standard_name String mass_concentration_of_alpha_carotene_in_sea_water
attribute alpha_carotene units String kg/m^3
variable alloxanthin   double  
attribute alloxanthin long_name String Alloxanthin
attribute alloxanthin units String kg/m^3
variable antheraxanthin   double  
attribute antheraxanthin long_name String Antheraxanthin
attribute antheraxanthin units String kg/m^3
variable beta_carotene   double  
attribute beta_carotene long_name String Beta Carotene
attribute beta_carotene standard_name String mass_concentration_of_beta_carotene_in_sea_water
attribute beta_carotene units String kg/m^3
variable c2mgdg   double  
attribute c2mgdg long_name String C2MGDG
attribute c2mgdg units String kg/m^3
variable chl_a   double  
attribute chl_a long_name String Concentration Of Chlorophyll In Sea Water
attribute chl_a standard_name String concentration_of_chlorophyll_in_sea_water
variable chl_b   double  
attribute chl_b long_name String Chlorophyll-B
attribute chl_b standard_name String mass_concentration_of_chlorophyll_b_in_sea_water
attribute chl_b units String kg/m^3
variable chl_c1   double  
attribute chl_c1 long_name String Chlorophyll-C1
variable chl_c1_c2   double  
attribute chl_c1_c2 long_name String CHL C1 C2
attribute chl_c1_c2 units String kg/m^3
variable chl_c2   double  
attribute chl_c2 long_name String Chlorophyll-C2
attribute chl_c2 units String kg/m^3
variable chl_c3   double  
attribute chl_c3 long_name String Chlorophyll-C3
attribute chl_c3 standard_name String mass_concentration_of_chlorophyll_c3_in_sea_water
attribute chl_c3 units String kg/m^3
variable chl_ide_a   double  
attribute chl_ide_a long_name String Chl Ide A
attribute chl_ide_a units String kg/m^3
variable chla_allomer   double  
attribute chla_allomer long_name String Chlorophyll-A Allomer
attribute chla_allomer standard_name String concentration_of_chlorophyll_in_sea_water
attribute chla_allomer units String kg/m^3
variable chla_prime   double  
attribute chla_prime long_name String Chlorophyll-A prime
attribute chla_prime units String kg/m^3
variable diadinoxanthin   double  
attribute diadinoxanthin long_name String Diadinoxanthin
attribute diadinoxanthin standard_name String mass_concentration_of_diadinoxanthin_in_sea_water
attribute diadinoxanthin units String kg/m^3
variable diatoxanthin   double  
attribute diatoxanthin long_name String Diatoxanthin
attribute diatoxanthin units String kg/m^3
variable dv_chl_a   double  
attribute dv_chl_a long_name String DV Chlorophyll-A
attribute dv_chl_a units String kg/m^3
variable fuco   double  
attribute fuco long_name String Fuco
attribute fuco standard_name String mass_concentration_of_fucoxanthin_in_sea_water
attribute fuco units String kg/m^3
variable gyroxanthin_diester   double  
attribute gyroxanthin_diester long_name String Gyroxanthin Diester
attribute gyroxanthin_diester units String kg/m^3
variable lutein   double  
attribute lutein long_name String Lutein
attribute lutein standard_name String mass_concentration_of_lutein_in_sea_water
attribute lutein units String kg/m^3
variable me_chlide   double  
attribute me_chlide units String kg/m^3
variable mgdvp   double  
attribute mgdvp units String kg/m^3
variable neoxanthin   double  
attribute neoxanthin long_name String Neoxanthin
attribute neoxanthin units String kg/m^3
variable peri   double  
attribute peri long_name String Peri
attribute peri units String kg/m^3
variable phbide   double  
attribute phbide long_name String Phbide
attribute phbide units String kg/m^3
variable phphyt   double  
attribute phphyt long_name String PHPHYT
attribute phphyt units String kg/m^3
variable prasinoxanthin   double  
attribute prasinoxanthin long_name String Prasinoxanthin
attribute prasinoxanthin standard_name String mass_concentration_of_prasinoxanthin_in_sea_water
attribute prasinoxanthin units String kg/m^3
variable violaxanthin   double  
attribute violaxanthin long_name String Violaxanthin
attribute violaxanthin standard_name String mass_concentration_of_violaxanthin_in_sea_water
attribute violaxanthin units String kg/m^3
variable zeaxanthin   double  
attribute zeaxanthin long_name String Zeaxanthin
attribute zeaxanthin standard_name String mass_concentration_of_zeaxanthin_in_sea_water
attribute zeaxanthin units String kg/m^3
variable total_chl_a   double  
attribute total_chl_a long_name String Concentration Of Total Chlorophyll-A In Sea Water
attribute total_chl_a units String kg/m^3
variable all_chl_a   double  
attribute all_chl_a long_name String Concentration Of All Chlorophyll-A In Sea Water
attribute all_chl_a units String kg/m^3
variable all_chl_a_flag   String  
attribute all_chl_a_flag long_name String All Chl A Flag
variable analyzing_lab   String  
attribute analyzing_lab long_name String Analyzing Lab
variable row_flag   String  
attribute row_flag long_name String Row Flag
variable quality_level   String  
attribute quality_level long_name String Quality Level
variable comments   String  
attribute comments long_name String Comments
variable quality_log   String  
attribute quality_log long_name String Quality Log

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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